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娌沖彛楸肩被鏄渤鍙ｇ敓鎬佺郴緇熺殑閲嶈緇勬垚閮ㄥ垎錛屽湪浜虹被媧誨姩鏃ョ泭鍔犲墽鐨勮儗鏅笅錛岄奔綾誨鏍鋒�у拰緹よ惤緇撴瀯鐨勭洃嫻嬭秺鏉ヨ秺鍑告樉鍏墮噸瑕佹�с�傜劧鑰屾渤鍙ｆ搗鍩熸按娣辮緝嫻呫�佽埅綰胯緝澶氬拰姘翠綋嫻戞祳絳夛紝鐜版湁鐨勪竴浜涜皟鏌ユ墜孌靛父甯稿彈鍒頒竴浜涢檺鍒躲�傜爺絀跺洟闃熷湪涔嬪墠寤虹珛鐨勬渤鍙ｆ搗鍩焑DNA瀵岄泦鏂規(guī)錛圖OI: 10.24272/j.issn.2095-8137.2021.331錛汥OI: 10.12131/20210304錛夊拰鐝犳睙娌沖彛鏈簳楸肩被eDNA瀹忔潯褰㈢爜鏁版嵁搴擄紙DOI: 10.12131/20210210錛夌殑鍩虹涓婏紝鍒╃敤eDNA瀹忔潯褰㈢爜鐮旂┒浜?jiǎn)鐝犳睙鍙ｄ级浠儖z嬬殑楸肩被鐗╃緇勬垚銆佺兢钀芥椂絀哄樊寮備互鍙?qiáng)涓庣幆澧冨洜瀛愮殑鍏尘p伙紝騫朵笌搴曟嫋緗戣皟鏌ユ柟娉曡繘琛屼簡(jiǎn)姣旇緝銆?/p>
璇ョ爺絀朵簬縐嬪鍜屾槬瀛ｅ湪鐝犳睙娌沖彛鐨?0涓洃嫻嬬偣鍚屾椂榪涜浜?jiǎn)搴曟嫋缃戞崟鎹炪�佹按浣撻噰鏍峰拰鐜鍥犲瓙嫻嬮噺銆傛按浣撴牱鏈繃婊ゅ悗鎻愬彇eDNA錛屽埄鐢∕iFish-U/E寮曠墿榪涜綰跨矑浣?2SrRNA鍩哄洜錛堢害172bp錛夌殑鎵╁銆佸緩搴撱�佹祴搴忓拰OTU娉ㄩ噴錛岄�氳繃鏁版嵁搴撶殑姣斿鑾峰緱楸肩被鐗╃緇勬垚銆備富瑕佺粨鏋滄湁錛?. eDNA鏂規(guī)硶鍦ㄦ瘡涓噰鏍風(fēng)偣鍧囨瘮搴曟嫋緗戞槑鏄炬鍑烘洿澶氱殑鐗╃錛屾�諱綋涓奺DNA鍏辨鍑?14縐嶉奔綾伙紙搴曟嫋緗?0縐嶏級(jí)錛屽悓鏃秂DNA媯�(gè)嫻嬬綾葷殑鐩稿涓板害涓庨奔綾葷墿縐嶄赴搴?鐢熺墿閲忎箣闂村瓨鍦ㄦ樉钁楃浉鍏蟲(chóng)�с�傞奔綾葷敓鎬佷範(fàn)鎬у垎鏋愯〃鏄庯紝eDNA鍙互媯�(gè)鍑哄洜浼犵粺娓旂綉緗戠洰澶у皬闄愬埗鑰屾紡媯�(gè)鐨勫皬鍨嬮奔綾伙紝浣塊奔綾葷兢钀界敓鎬佺壒寰佹洿鍔犲畬鏁達(dá)紱2. 涓ょ鏂規(guī)硶鍧囨嫻嬪埌楸肩被縐嶇被鐨勫鑺傛洿鏇匡紝鍗蟲(chóng)槬瀛ｅ拰縐嬪楸肩被緹よ惤瀛樺湪鏄捐憲宸紓錛宔DNA鏂規(guī)硶媯�(gè)嫻嬪埌鐨勬樉钁楁�ф瘮搴曟嫋緗戞洿寮恒�?. eDNA涓庡簳鎷栫綉涓�鏍峰潎鎻ず浜?jiǎn)楸季c葷兢钀界粍鎴愬樊寮傞殢鐫�鍦扮悊璺濈鐨勫鍔犺�屾樉钁楀鍔犮�備笂榪扮粨鏋滆〃鏄庡湪娌沖彛鐢熸�佺郴緇熶腑錛岀壒鍒槸浼犵粺璋冩煡鏂規(guī)硶瀛樺湪鍥伴毦鐨勬儏鍐典笅錛宔DNA瀹忔潯褰㈢爜鍙綔涓轟竴縐嶉噸瑕佺殑琛ュ厖鎵嬫鐢氳嚦鏇夸唬鏂規(guī)硶搴旂敤浜庨奔綾葷兢钀界殑瀛ｈ妭鎬у彉鍖栧拰絀洪棿鍒嗗竷鍙樺寲鐨勭洃嫻嬶紝鎻愬崌璇勪及娌沖彛鐢熸�佺郴緇熶腑楸肩被緹よ惤緇撴瀯鐨勮兘鍔涳紝鍦ㄦ笖涓氱爺絀墮鍩熷拰鐢熸�佺幆澧冪洃嫻嬮鍩熷叿鏈夊緢濂界殑搴旂敤鍓嶆櫙銆?/p>
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璁烘枃閾炬帴錛?a >https://www.sciencedirect.com/science/article/pii/S1470160X22012274
鍩轟簬eDNA鍜屽簳鎷栫綉鐨勯奔綾葷墿縐嶇粍鎴愬姣?/p>
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綺ゅ叕緗戝畨澶?4010502001742鍙?/a>