鍗楁搗鎵璇勪及鐜DNA錛坋DNA錛夋妧鏈簲鐢ㄤ簬椴哥被鐨勭洃嫻嬬爺絀跺彇寰楁柊榪涘睍
榪戞棩錛屼腑鍥芥按浜х瀛︾爺絀墮櫌鍗楁搗姘翠駭鐮旂┒鎵鐝嶇█嬋掑嵄鍔ㄧ墿淇濇姢鍥㈤槦鍦ㄨ瘎浼扮幆澧僁NA錛坋DNA錛夋妧鏈椴哥被鐩戞祴鏂歸潰鐨勫簲鐢ㄧ爺絀跺彇寰楁柊榪涘睍錛屽彂琛ㄤ簡鍥藉唴絎竴綃囨搗媧嬮哺綾籩DNA鐩戞祴鐮旂┒璁烘枃銆傝鍥㈤槦鑱斿悎鍗椾含甯堣寖澶у絳夊崟浣嶇鐮斾漢鍛橈紝浠ユ繁鍦沖ぇ楣忔咕嫻峰煙鍑虹幇鐨勫皬甯冩皬椴鎬負渚嬶紝鎺㈣浜唀DNA鍦ㄨ幏鍙栭哺綾葷墿縐嶄俊鎭侀鐗╄祫婧愮粍鎴愩佹棭鏈熼璀﹀拰榪佺Щ璺嚎絳夋柟闈㈢殑搴旂敤銆傝鐮旂┒鎴愭灉浠モ?span style="font-family: times new roman;">Assessing the potential use of environmental DNA for multifaceted genetic monitoring of cetaceans: Example of a wandering whale in a highly disturbed bay area鈥濅負棰樺彂琛ㄥ湪鍥介檯鐢熸佸棰嗗煙鏈熷垔Ecological Indicators錛圝CR Q1錛変笂錛堝紶甯呭崥澹負絎竴浣滆咃紝鏉庢晱鍗氬+涓洪氳浣滆咃級銆?/p>
椴哥被鏄叏鐞冩渶鍙楀▉鑳佺殑鑴婃鍔ㄧ墿綾葷兢涔嬩竴錛屾湁37%鐨勭墿縐嶅浜庡彈濞佽儊鐘舵併傜洰鍓嶉哺綾葷殑鐩戞祴宸ヤ綔浠嶉潰涓村閲嶆寫鎴橈紝鐢變簬鍏舵湰韜暟閲忕█灝戙佷笖鍏惰涓洪毦浠ユ崏鎽革紝鍔犱箣浼犵粺鐮旂┒鏂規硶鐨勯檺鍒訛紝璁稿椴哥被鐨勫垎甯冧俊鎭粛澶勪簬絀虹櫧鐘舵侊紝榪欐瀬澶ч樆紕嶄簡淇濇姢琛屽姩鐨勫紑灞曘?/p>
鍗楁搗鎵鐮旂┒鍥㈤槦閫氳繃閲囬泦嫻鋒按鏍鋒湰錛屽埄鐢╡DNA鎶鏈2021騫?鏈堝簳鍑虹幇鍦ㄦ繁鍦沖ぇ楣忔咕鐨勪竴澶撮』椴革紙鏄電О鈥滃皬甯冣濓級榪涜浜嗙綾婚壌瀹氥傞氳繃鑾峰彇鐨?涓嚎綺掍綋鍩哄洜搴忓垪鐗囨錛岃瘉瀹炲叾灞炰簬甯冩皬椴歌繎宀鎬簹縐嶏紙Balaenoptera edeni edeni錛屽皬甯冩皬椴告垨槌椴革級錛屽茍琛ㄦ槑鍩轟簬閫氱敤寮曠墿鎵╁鐨勫畯鏉$爜嫻嬪簭鍜屽熀浜庣墿縐嶇壒寮傚紩鐗╂墿澧炵殑Sanger嫻嬪簭鍧囧彲鐢ㄤ簬椴哥被鐨別DNA鐗╃閴村畾銆?/p>
鍥? 嫻蜂笂瑙傛祴鍜宔DNA閲囬泦
鐮旂┒鍥㈤槦閫氳繃鍥炴函姝ゅ墠鍦ㄩ偦榪戞搗鍩燂紙鐝犳睙鍙e拰澶т簹婀撅級榪涜鐨勬搗媧嬬敓鐗〆DNA甯歌鐩戞祴緇撴灉錛堝浘2錛夛紝鎰忓鍦板彂鐜板湪澶ч箯婀鋸滃皬甯冣濊鍙戠幇鐨勪袱涓鏈堝墠錛?021騫?鏈堬級錛屽湪鐝犳睙鍙e嫻峰煙灝辨嫻嬪埌浜嗗皬甯冩皬椴哥殑瀛樺湪銆傜粨鍚堝凡鏈夌殑鐮旂┒鍒嗘瀽鎺ㄦ祴錛氣滃皬甯冣濆彲鑳芥潵鑷稜媧插矝緹や綋錛屼絾鍚屾椂涓嶆帓闄ゅ叾鏉ヨ嚜涓滃崡娌挎搗鐨勫叾浠栨湭鐭ョ兢浣撱?/p>
鍥? 澶ч箯婀懼強閭昏繎嫻峰煙eDNA鐩戞祴浣嶇偣
鐮旂┒鍥㈤槦榪樺埄鐢╡DNA鏍鋒湰鍜?2S鎵╁瀛愰珮閫氶噺嫻嬪簭錛屽悓鏃惰В鏋愪簡澶ч箯婀炬搗鍩熺殑楸肩被縐嶇被緇勬垚鍜岀浉瀵逛赴搴︼紝緇撳悎鐜板満鏀墮泦鐨勨滃皬甯冣濇崟椋熼奔綾繪牱鏈紝鍙戠幇鍏舵綔鍦ㄧ殑椋熺墿涓昏鏄矙涓侀奔綾誨拰槌楸肩被錛堝浘3錛夈傞氳繃涓庨偦榪戞搗鍩熼奔綾葷兢钀界粨鏋勭殑瀵規瘮錛堝浘4錛夛紝緇撳悎灝忓竷姘忛哺鐨勫彂鐜版搗鍩熷拰鏃墮棿錛屾帹鏂叾寰堟湁鍙兘鏄拷韙繖浜涙磩娓哥殑楸肩被榪涘叆澶ч箯婀俱?/p>
鍥? 鍩轟簬鐜板満鏍鋒湰鍜宔DNA鍒嗘瀽鐨勫ぇ楣忔咕灝忓竷姘忛哺娼滃湪鎹曢楸肩被
鍥? 鍩轟簬eDNA瑙f瀽涓変釜嫻鋒咕鐨勯奔綾葷兢钀界粨鏋勫樊寮?/span>
璇ョ爺絀惰〃鏄庯紝eDNA鎶鏈綔涓轟竴縐嶉珮鐏墊晱鎬у拰鍑嗙‘鎬с佷綆鎵板姩鐨勨滈潪鍏ヤ鏡鈥濈爺絀舵墜孌碉紝鍗佸垎閫傚悎浜庣弽紼嬋掑嵄鍔ㄧ墿鐨勭洃嫻嬶紝鍦ㄩ哺綾葷綾婚壌瀹氥侀鐗╄儗鏅俊鎭皟鐮斻佹棭鏈熼璀︾洃嫻嬩互鍙婃磩娓?榪佺Щ璺嚎鐮旂┒絳夋柟闈㈠叿鏈夎壇濂界殑搴旂敤鍓嶆櫙銆?/p>
璇ョ爺絀跺緱鍒板浗瀹剁鎶鍩虹璋冩煡涓撻」錛?019FY101900錛夈佸啘涓氬啘鏉戦儴娓斾笟鍙戝睍琛ュ姪璧勯噾鈥滅弽璐墊繏鍗辨按鐢熼噹鐢熷姩鐗╂爾鎭湴璋冩煡鈥濆拰騫夸笢鐪佺鎶璁″垝欏圭洰錛?019B121201001錛夌瓑欏圭洰璧勫姪銆?/p>
璁烘枃閾炬帴錛?a >https://www.sciencedirect.com/science/article/pii/S1470160X23002674